A novel, simple, precise and accurate area under curve spectroscopy method has been developed for estimation of capecitabine in bulk and pharmaceutical dosage form and AUC was measured at 301-311nm in methanol: acetonitrile: distilled water (70:20:10 v/v) and obeys Beer’s law in the concentration range of 2-10 µg/ml. The linearity study was carried and regression coefficient was found to be 0.9999 and it shows good linearity, precision in this concentration range. The regression equation was found to be Y = 0.0294X + 0.0007. The % recovery was found to be 99.85-100.39. The LOD and LOQ were found to be 0.055 and 0.165 µg/ml. The % relative standard deviation was found with in ± 2. The method has been validated according to ICH guidelines for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed validated method was successfully applied for reliable quantification of Capecitabine in bulk and pharmaceutical dosage form.
Loading....